Stereochemistry of spermidine synthase
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چکیده
منابع مشابه
Specificity of mammalian spermidine synthase and spermine synthase.
1. The specificity of rat prostatic spermidine synthase and spermine synthase with respect to the amine acceptor of the propylamine group was studied. 2. Spermidine synthase could use cadaverine (1,5-diaminopentane) instead of putrescine, but the Km for cadaverine was much greater and the rate with 1mM-cadaverine was only 10% of that with putrescine. 1,3-Diaminopropane was even less active (2% ...
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Experiments with D-glucose-6-P stereospecifically tritiated at C-6 showed that the myo-inositol-1 P synthase reaction catalyzed by both the enzyme from beef testis and from pollen of Lilium longiflorum proceeds with stereospecific loss of the pro-6R and incorporation of the pro-6S hydrogen into the product. The ring closure thus occurs in a retention mode at C-6 of the substrate, a finding at v...
متن کاملSpermidine synthase of Escherichia coli: localization of the speE gene.
We have obtained Escherichia coli mutants lacking spermidine synthase (putrescine aminopropyltransferase) and have found that the mutated gene (speE) is located immediately upstream from the gene coding for S-adenosylmethionine decarboxylase (speD); these genes are located at 2.7 minutes on the E. coli chromosome. Both genes are present in a 1795-base-pair fragment of E. coli DNA that was clone...
متن کاملGenetic analysis of spermidine synthase from Leishmania donovani.
The polyamine biosynthetic pathway of protozoan parasites has been validated as a target in antiparasitic chemotherapy. To investigate this pathway at the biochemical and genetic level in a model parasite, the gene encoding spermidine synthase (SPDSYN), a key polyamine biosynthetic enzyme, has been cloned and sequenced from Leishmania donovani. The L. donovani SPDSYN gene encodes a polypeptide ...
متن کاملSpermidine synthase is required for virulence of Leishmania donovani.
Genetic lesions in the polyamine biosynthetic pathway of Leishmania donovani, the causal agent of visceral leishmaniasis, are conditionally lethal mutations that render the insect vector form of the parasite auxotrophic for polyamines. Recently, we have demonstrated that a Δodc L. donovani null mutant lacking ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis, was...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 1982
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja00385a057